Hey everybody, I have tried to create a macro for my image analysis but thus far I didn’t figure out the necessary tool for it.
I have tissue sections with stained cells and want to randomly pick a specific amount in a specific region after marking all of them so that I can analyse them later.
So the idea is to mark all cells of the area (blue frame) and then randomly but somewhat distributedly get about 20 cells to analyse (the marking beforehand would happen so that I can assure that only whole cells are counted as sometimes the signal comes from processes only and not from cell bodies).
Is there a way to do this? Most randomization scripts I have found so far use rectangular ROIs and I wouldn’t know how to use them for this project.