I used Ventana DP200 tissue scanner from Roche to digitalize several DAB slides. I performed Z-Stack scanning and obtained .bif images. Although Roche native software allows to visualize the images and their different optical slices in Z, no other program is able to open files in this format (.bif) or to show the Z-Stack options. Specifically, QuPath opens the files showing only one optical slice and doesn’t detect any other slice (showing always “series 0”). I tried importing with BioFormats, I tried FIJI, Huygens but none of them worked. I found a web viewer called PMA.start which is able to open these Z-Stacks, however, it does not allow to export good-quality snapshots. If anyone has an idea to open .bif whole slide images in QuPath I’ll be glad to hear it.
Hint: In PMA.start I discovered that each .bif file has a corresponding .tifp hidden file that is necessary to open the Z-stack properly.