I’m running cell detection in QuPath and I see differences when I run it on a full image vs. a cropped region:
It detects additonal cells in the full image, even though the same local information is present in the cropped image; they’re both 8-bit and I did not re-scale intensity or anything like that.
Cell detection settings:
requested pixel size: 0
median filter: 0
min. area: 10
max. area: 75
include nucleus: yes
Any ideas why this occurs and how I can ensure consistent results? Thanks!