Hey QuPath Community,
I have recently started using QuPath for analyzing tendon tissue histology slide images and I am attempting to quantify the following objects and features:
collagen alignment - organized vs unorganized
Rounding of Nuclei
amount of collagen
vascularity (if present)
I have watched several very informative videos from Pete Bankhead’s YouTube playlist and have been able to implement the onboard cell detection method as well as the onboard ImageJ analysis tools but would like to be more proficient with these tools.
I am working with large svs image files that are stained with Masson’s Trichrome:
The organized collagen would be the concentrated blue pieces up top and the unorganized would be the lighter blue that is slightly below that has a larger amount of cells in it.
Any and all help/tips is greatly appreciated!