I am using the latest milestone 6 for my image analysis.
I am trying to analyze fluorescent images taken on delta vision systems. I am staining for 3 surface markers and DAPI stain. Below are the jpeg images of representative sample image.
First I tried to segment the DAPI channel for getting the cells by expanding the nucleus detection. Then using the intensity measurements within the cells to define positive cells in other channels. Since all are surface markers in my images, so I don’t get exact detections.
In order to overcome this I am trying to segment all the channels separately and want to do colocalization to get single, double or triple positives. But in qupath, I can segment only one channel and as I segment the second channel, I loose the segmentation for first channel.
I will appreciate any suggestions in this regard or even any other suggestion for this kind of image analysis.