I’m new to CellProfiler and need some help.
I am working to count the number of arbuscules( like the dark parts in the picture )
in root samples for a study calculating the percent colonisation of roots. I want to identify and count the number of arbuscules, I tried DLpipe that identifying the stained parts or particles, but it worked not well. It is hard to distinguish the dark parts from other parts of the picture. The pipeline I tried is below. Can you tell me how can I do it with CellProfiler?
Many thanks in advance,
DLpipe.cppipe (13.0 KB)