I have some questions about the heat map function.
Firstly, what the function of the “Geometry/length Major Axis”? If I want to measure the cell length of width, should I run this function or following the “Bezier point” function or PCA tools?
Secondly, for the “shape analysis”, I have no idea about the "Custom X or Y " function. Meanwhile, when I run the 3D PCA function to analyze 3D cell mesh, sometimes it is not workable, but it worked with 2D PCA. Is there no difference between them? Thanks in advance.