I’m pretty new in image analysis, and for my project i have to quantify the amount of Mucin (stained blue) and compare it to the amount of inflamed epithelial tissue (stained in pink).
I already have a pretty good method for quantifying the Mucin by colour deconvolution, but the problem lies with the pink quantification: I need only the epithelial tissue, not the Muskularis that surrounds it. (see example image)
I already tried using the weka trainable segmentation, but unfortunately the images (even when i downsize them to 10% of their original size) are too big, therefore fiji can’t keep up. Is there any other way to segment those different tissues?
Thank you in advance!