Quantifying two types of areas of multiplex image with cytokeratin

Hello all,
I have a multiplex tumor tissue sample with cytokeratin and other immune cell markers. But I want to use qupath to automatically via algorithm or machine learning to detect tumor vs. stroma as two different categories. From the two categories of tumor vs. stroma, I want to detect and quantify individual cell markers from the multiplex. would it be possible to use Qupath?


It really depends on your markers and your sample. There is some information here, where the tumor area was defined by the cytokeratin marker. Multiplex doesn’t necessarily mean IF, though, so your options may be different if you have a brightfield multiplex.

Ok great!! Thank you so much Research_Associate!

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