Hello, dear colleagues,
I need help with some really simple task like for QuPath:
I have an experimental lung from mouse with metastatic melanoma. I wanna measure how much µm² and also “%” tumor and normal tissue i have.
I tried to do it with pixel classifyer, but the resuöts didn’t made me happy, because:
- alveoli in the lung are transparent and dont have nucleated cells - thais way it wasn’t detected like tissue;
- with pixel classifyer i have a lot of cell parameter in “measure”, but i didn’t found “totally area”
This reason i marked tumor areas and lung manually with “wall” and try to calculate this with help of “measure” - “show annotation measurements”… but i have the feeling, that % with QuPath are too low, for example on the microscope i give 25% and QuPath numbers give me just 10%. I think that this is big differences.
Qould you please tell me, or QuPath measure just pixel size or some more parameter? Is it possible to perform my measures in another way?
Thank you in advance - i add pictures in comment