I need to analyse some fluorescent confocal images stained with double presynaptic markers and other images stained with pre- and postsynaptic markers. In order to do so, I was told to try the ImageJ plugin “Puncta Analyzer”. However, I’m experiencing difficulties with it, and these are the same that a coupled of years ago started a discussion in ImageJ’s mailing list http://imagej.1557.x6.nabble.com/Puncta-Analyzer-td4892020.html
I’ve managed to install Puncta-Analyzer v1.1 in Fiji-ImageJ (ImageJ v1.51a; Java 1.8.0_66), but couldn’t get it to show nor save any results. I then ran the plugin in an older version of ImageJ (1.46r; Java 1.8.0_66), only to obtain the same exception:
java.lang.NoSuchMethodError: ij.ImagePlus.getMask()[I at Puncta_Analyzer$ScopeImageUtils.contractHistogram(Puncta_Analyzer.java:708) at Puncta_Analyzer.locatePunctaInColorBand(Puncta_Analyzer.java:660) at Puncta_Analyzer.locatePuncta(Puncta_Analyzer.java:620) at Puncta_Analyzer.run(Puncta_Analyzer.java:240) at ij.plugin.filter.PlugInFilterRunner.processOneImage(PlugInFilterRunner.java:256) at ij.plugin.filter.PlugInFilterRunner.<init>(PlugInFilterRunner.java:105) at ij.IJ.runUserPlugIn(IJ.java:187) at ij.IJ.runUserPlugIn(IJ.java:196) at ij.IJ.runPlugIn(IJ.java:152) at ij.Executer.runCommand(Executer.java:127) at ij.Executer.run(Executer.java:64) at java.lang.Thread.run(Thread.java:745)
Since in the discussion I mentioned, the possibility of fixing this issue with Punta Analyzer was considered, maybe by developing a Puncta-Analyzer v2, I was wondering whether something was advanced in this sense. I believe that, although quite some time has already passed since then, @ctrueden may know something about it.
Thank you all in advance.
Carlos Pretell Annan