Quantifying P53 and DAPI Levels

Hi Guys,

Im quite new to all this and Im currently doing some research trying to quantify P53 levels in different fluorescent staining photomicrographs. I have already taken the images and they show both DAPI and P53 levels. How can I quantify each accurately on a batch of 5/6 images.

I kindly appreciate anyones help.

Kind Regards,

Sam










Hi Sam,

I’d recommend taking a look at our example pipelines page, and see if any of the pipelines there come close to doing what you want; the “Cell/particle counting, and scoring the percentage of stained objects” one seems most similar at first glance.

Regards,
-Mark