I am trying to quantify cell orientation by measuring the nuclei to golgi angle in endothelial cells. At the moment, I am doing it manually and so can only do about a 750-1000 cells per sample. I have images containing ~10000 cells and would like to automate this process to expand my analysis.
I have included a sample image where golgi is green and nuclei are blue.
Any suggestions on how to achieve this? I have a basic understanding of ImageJ macros and segmentation.
A1.tif (2.9 MB)