I am looking for a way to measure microtubule distribution in cells for comparison with cells in other treatment groups.
I have tried using FIJI to pre-process my images (Subtracting background, thresholding, Difference of Gaussian) and running plugins such as ridge detection to find the filaments, but I can’t seem to find a method that will allow for good quantification or replication.
My thought process was to segment the cells, capture the filaments, and find a way to calculate the area of the filaments that lie within the segmented cells, but I have not had much luck with this.
I’d greatly appreciate any suggestions for methods of quantification, or information about other programs (CellProfiler maybe?) or workflows/pipelines that could accomplish this.
Attached below are two microscopy pictures that I want to compare.