Hi there, I’m trying to quantify fluorescence using the 3D ROI manager and I’m run into several issues. I first use segmentation editor to select my ROIs across slices and interpolate between. For each brain, I have 2 ROIs (left and right) isolated across slices. After saving an ROI, I open 3D ROI Manager so I can quantify fluorescence within each of my ROIs. The biggest issue is that 3D ROI Manager only works some of the time. Most of the time, it freezes as soon as I try to add an image. Sometimes I can add one ROI, but I can’t add the other. I have over 100GB of memory available and 8GB of RAM. Should I upgrade my RAM to get the 3D Manager to work, or am I doing something wrong?
Thank you SO much in advance!!