Hello. I am very new to Fiji and image analysis. I want to find out what my options are for quantifying differences in cell types and thicknesses of fish epidermis and dermis in fish that are being used for a wound-healing experiment using cleaner shrimps. Fish are being processed for standard H&E histology to identify normal tissue, initial wound initiation (removal of scales from the epidermis), and 2, 4 and 7 days post wound initiation to detect healing.
To begin with I would like to use Fiji in a pilot study to determine the variation in a sample of 10 individual fish to confirm that my sample size is good. Thereafter, I will begin the experiment.
Can anyone offer me any advice please on a simple method for quantifying the different cell types and thicknesses of regions in a standard H&E section of fish skin? Are there any protocols that have similarly been written for this that I could use and practice with to start? My knowledge of image analysis is currently very basic.
Any help is greatly appreciated.
Yours sincerely, David