I am trying to measure the whole cell fluorescence intensity using DAPI channel defined ROI.
This is what I did:
Define the ROI using “Analyze Particles” function in the DAPI (nucleus) channel.
I used this defined ROI to read the fluorescence intensity in other channels. So of course, these other signals should be all within the nucleus region.
Q: So now I want to have the intensity for the non-nucleus region but still have the defined cell numbering (see the pic above). I have tried the segmentation function, or binary then erode the boundary a little bit, but both are not very ideal. I am wondering if anyone has a better idea to do this? Real Appreciate!
The original file attached with the zip file here (https://www.dropbox.com/s/v3feu7bk0kucfil/Image%20108.czi?dl=0), pls help to have a look, thanks!