Hello, I am new to CellProfiler but have a couple of questions regarding scoring fluorescent objects.
I have followed the example on the CP website to find the percentage of positive nuclei vs total number of nuclei using the CalculateMath Module. However, this example assumes that a DNA stain (i.e. DAPI) was used to help segment individual cells.
Is there a way in which I can determine the percentage of nuclei expressing GFP in a population of cells without using a channel for a DNA stain (since I did not use one)?
I would like nuclei to be filtered according to the property of having GFP (positive) or not having GFP (negativeobject) related to them.
Also, can this be done using bright field images?
Thank you in advance!