Hello everyone. I am a new user of ImageJ. We are interested in measuring fluorescence intensity/background of stained cells. This is how I did the analysis:

- Split the picture into green channel.
- Select individual cells by drawing a straight line through it, then obtain the plot profile. From there, I get the gray value and calculate the ratio.

The problem for this is only few cells can be selected and the ratio is cell-dependent as some cells are brighter than others. If we want to get a more accurate measurement based on the whole picture, will anyone be able to help?