CCC3.VK15_5.0x(1).tif (10.8 MB)
Hello,
This image is a coronal view of a mouse skull. In the middle, the bone has been removed surgically. This created a defect. Now, we want to know how much bone has been repaired within the defect area. We also want to qualify this new bone by assessing the trabecular surface (porosity of new bone = surface of hole within this new bone).
I plan to :
- Quantify the amount of new bone (in black surface) within the bone defect (not in the side where the native skull is).
Could you please provide me a protocol that includes :
- the creation of a ROI
- the calculation of the amount of black surface / ROI total surface.
- repeat this procedure on several images (batch)
- Qualify the new bone by assessing the surface of trabeculation (porosity = surface of hole) within the new bone formation in the ROI
Could you please provide me a protocol that includes :
- the creation a ROI
- the calculation of the surface of white/grey holes within the bone in the ROI / total surface of bone in the ROI
- repeat this procedure on several images (batch)
Thanks very much
MB) DDD2.VK8_5.0x(1).tif (10.8 MB)