I have TEM images of mitochondria, and I am trying to quantify them based off several parameters like number, size, and circularity using ImageJ. I have been reading about the macro developed by Dagda (https://imagejdocu.tudor.lu/plugin/morphology/mitochondrial_morphology_macro_plug-in/start), and it seems promising. However, we have not used the MitoTracker stain for our images so the macro does not work properly. I don’t have much experience with using ImageJ, so would anyone be able to push me in the right direction? Thank you!