I don’t use ImageJ very often but I am currently trying to use it to quantify fluorescent images from IF. My staining is quite punctate. I have seen another paper published which uses the interactive 3D surface plot viewer to better visualise the intensity of the staining between samples, and I have been able to do this. However, I was wondering if there was a way to quantify the plot? If so, can this further be applied to selecting individual cells, creating a 3D plot, and then quantifying this?