I am a beginner in image processing analysis and I have too many free software but I want an advice on the scientific way to quantify the fluorescence in different images (like the attached)
Do I have to split channels?
what is the best image channel ? why?
can I perform the analysis on RGB images of I have to convert it to a grayscale?
too many questions ?
but to give a small hint, I want to make a relation between different concentrations with different fluorescence so I want to determine the fluorescence intensity for each concentration.