I am trying to quantify DAB stained area on leaves by using trainable Weka segmentation.
I am now in a dilemma, whether i need to adjust my contrast and brightness(for easier segmentation) or not.
I have tried segmenting using original and modified image, and both of these give different result.
In order to get ground truth result, do i need to use original image or modified image?
Another problem i have faced is: classifier trained by using only one sample image is not enough as the other leaves have different intensity of stain. Once the classifier is trained, i could not train it again by using other sample image.
Thank you in advance!