I am very interested in using CellProfiler to quantitate the cytoplasmic foci (GW bodies or p-bodies) that our lab are mainly interested in. One
basic question is whether these cytoplasmic foci can be quantitated by CellProfiler.
Green staining is anti-GW182, one of the major components of GWBs. The number and size of these cytoplasmic dots are our main focus. Is it fessible to tell the difference in number and size of these foci between the two images?
Please let me know your opinions. Your help is highly appreciated! Thanks a lot!