WTAA2 (2’)_5.0x.tif (8.5 MB)
This image is a coronal view of a mouse skull. We want to assess the activity of bone remodelling by quantifying the amount of pink signal (TRAP staining) within the bone include in a certain ROI
Could you please provide me a protocol that includes :
- the creation of a ROI
- the calculation of the amount of pink signal in the bone within the ROI .
- repeat this procedure on several images (batch)
Thank you very much