I have developed a pipeline that identifies cells in rat brain tissues. It worked out pretty good and I got sizes and pixel intensity data from thousands of cells. I found that my treatment group had larger cell sizes and increased expression intensities. I am very excited to publish the data!
However, when I discuss the data with my boss, he concerned if anyone have published such data (quantifications of cell sizes and expression intensities etc) that were produced by using in vivo tissue images. In order to convince him I have been checking the citation list at the official Cell Profiler website, but couldn’t find one yet.
Can you recommend me any literatures using cell profiler to quantify cell sizes and intensities using in vivo tissue images (not in vitro culture system)?
Thanks! I really appreciate for your help!