i am working on device adaptor that can image and FRAP sequentially. i have noticed there is a projector plugin that i can utilise. However, as i understand the calibration of the projector plugin, it requires the illuminated spot from FRAP and imaging simultaneously. Therefore is there a way of using the same plugin to calibrate the FRAP without imaging at the same time? For example, detecting where the dark spots are after photobleaching, rather than looking for bright spots from FRAP?
Also, once the calibration is complete, where does the coordinates store? Is there a way to alter the calibration coordinates?