Processing + particle detection of TEM microvesicle images

Hi, I’d like to ask everyone’s opinion on processing TEM negative stain images of microvesicles. So far, I have found it impossible to get good automated quantification using the ‘quantify particles’ function. I wonder if there is a more sophisticated way, maybe specifying expected size of vesicles, or using the outlines of the vesicles rather than simple threshold setting to get better quantification. Any suggestions welcome! Thank you!

Hey @Nathalie_Javidi - have you looked into the Trainable Weka Segmentation tool? It’s a great plugin - pretty straightforward and powerful to use…

Also - check out this previous discussion:

Perhaps you’ll find it helpful. :smile: