Processing grayscale images?

I’ve been trying to use the yeast pipeline to process grayscale images. I don’t think CellProfiler pipelines can use grayscale images, at least the ones I’ve downloaded, so I would like to know if there is a way to modify the pipeline to work with grayscale. I mostly tried to change or even delete the ColortoGray step and then the Correct steps as well as other steps from RGB to other image types, but it didn’t seems to make the pipeline work. I’ve attached the image in question.


Most certainly CellProfiler can work with grayscale images. My guess is that in NamesAndTypes, you (or the pipeline you downloaded) have the setting “Select the image type” set to “Color image” rather than “Grayscale image”. If you have grayscale originals, then you likely have no need for the ColorToGray module.

If you continue to have trouble, feel free to post you pipeline along with this image.