I am working with mosaic pictures in zeiss format (zvi) and to work with them in Cellprofiler, I export them to tif files. There are 2 ways; I either state that I want them as a merged pictures or I get 12 mosaic pictures from each file. I prefer to use the merged version since it otherwise takes too long to process all the individual pictures, but I get an error message in the “CorrIlluminationCalcuate” module saying:
Error while processing CorrectIlluminationCalculate: Image must be grayscale but it was color.
I don’t get that error if I process the images unmerged. Very thankful for help!