Problems with measurements repeating

I am manually measuring cells using the measure tool. However I am finding certain measurements keep repeating in my data, which looks like:
25.338
24.324
18.243
22.297
15.203
14.189
21.284
19.257
16.216
20.27
21.284
19.257
24.324
25.338
28.378
24.324
23.311
18.243
19.257

As you can see I keep getting the same values repeating?

Thanks for any help you can offer.

Good day,

please show us an image and explain in detail what and how you are measuring.

Then please indicate the situation that leads to the same values.

Regards

Herbie

1 Like

Thanks for the response

I calibrate the image with a scale bar, then draw a straight line across the diameter of the cell with holding shift, and pressing control m to take the measurement. I click to remove the line and then measure the next cell.

On a discrete grid, there are a finite number of positions of the start and end pixels defining a line. Perhaps it is that what you are seeing.

I conjecture that you mean equal values for length-measurements.

I guess this is a sampling effect.
Because you use a scale, pixel spacings are mapped to micrometers and this mapping leads to decimal numbers not integers.

Now if you measure the same length in pixels you will get the correponding number in micrometers. If the objects are similar in size and are small, i.e. they measure only some tens of pixels,* it is quite likely that you get the same numbers.

HTH

Herbie


* According to your scale setting, the objects have diameters of around 40pixels and they are quite similar in size.

Ok that is clear… thanks for your help!