I am facing a rather odd problem in splitting Images in Cellprofiler. When I am using Input module to Load images into the pipeline, the splitting is not taking place but when I use Loadsingle Image module things are working fine.
Since I have huge data I was looking for Batch processing so can’t afford to use Loadsingle Image module.
Additionally, the images that I have are LSM images and I have converted them to Tiff images using Bioformats in ImageJ for CP analysis. (Used ImageJ for Batch conversion of images)
I did more thing, converted LSM image to tiff image using LSM image browser and surprising for this image, both Input module and Loadsingle Image are working.
I am very confused what is happening.
I am attaching the pipeline that I am using and also the images.
Test.cppipe (9.23 KB)