Hi! I want to share a problema with you. Maybe there is someone that has more experience doing macros with Image J and could help me or just give me some advices.
I’m working with fluorescent microscopy images that come from a time-lapse. What I want to do is create ROIs by stabling a threshold. Once I have created the mask, I overlap it in the image stack in order to measure the fluorescent inside this ROIs. I want to do this with so many videos so the macro will be so so useful for me.
WHAT I DO:
Try to create the macro. Plugins >> macros >> record. The recorder windows opens and I make the clicks and I see how the commands are recording. Then I save: create >> File >> save as…
Then I try to run de macro as: Plugins >> macros >> run
When I run the macro it doesn’t work well. It doesn’t select the same ROIs.
I have revised the recorded commands but I don’t know what is the problem. It seems all it’s already but the last step it’s not working well.
I don’t know what could be the problem or the solution. Maybe I need a plugin that I haven’t got yet.
Thanks in advance.