Problem with NucleusJ plugin

@ PouletAxel
@DesTristus

I am researcher at the IBENS in Paris, and really would like to use the Nucleus J plugin.
However, I have this error message when I try to use it:

(Fiji Is Just) ImageJ 2.1.0/1.53c; Java 1.8.0_66 [64-bit]; Windows 7 6.1; 903MB of 12242MB (7%)
 
java.lang.NullPointerException
	at gred.nucleus.segmentation.SegmentationCalling.<init>(SegmentationCalling.java:114)
	at gred.nucleus.plugins.NucleusSegmentationAndAnalysisPlugin_.run(NucleusSegmentationAndAnalysisPlugin_.java:45)
	at ij.IJ.runUserPlugIn(IJ.java:235)
	at ij.IJ.runPlugIn(IJ.java:198)
	at ij.Executer.runCommand(Executer.java:150)
	at ij.Executer.run(Executer.java:68)
	at java.lang.Thread.run(Thread.java:745)

I also tried Nucleus J 2.0 but it did not generate a cropped image nor any data.
I made sure that my images were in 8 bits. I up-dated Fiji, and dowload imagescience;
MorphoLibJ_-1.2.2; Jama-1.0.3 and the various version of the plugin but still it is not working.
Can anyone help me? The plugin looks exactly as what I need! I attached one of my sample image

Thanks a lot
Nathalie

C2-201217 Nucleus D17 Bio Actin 3-2.tif (2.4 MB)