I am researcher at the IBENS in Paris, and really would like to use the Nucleus J plugin.
However, I have this error message when I try to use it:
(Fiji Is Just) ImageJ 2.1.0/1.53c; Java 1.8.0_66 [64-bit]; Windows 7 6.1; 903MB of 12242MB (7%) java.lang.NullPointerException at gred.nucleus.segmentation.SegmentationCalling.<init>(SegmentationCalling.java:114) at gred.nucleus.plugins.NucleusSegmentationAndAnalysisPlugin_.run(NucleusSegmentationAndAnalysisPlugin_.java:45) at ij.IJ.runUserPlugIn(IJ.java:235) at ij.IJ.runPlugIn(IJ.java:198) at ij.Executer.runCommand(Executer.java:150) at ij.Executer.run(Executer.java:68) at java.lang.Thread.run(Thread.java:745)
I also tried Nucleus J 2.0 but it did not generate a cropped image nor any data.
I made sure that my images were in 8 bits. I up-dated Fiji, and dowload imagescience;
MorphoLibJ_-1.2.2; Jama-1.0.3 and the various version of the plugin but still it is not working.
Can anyone help me? The plugin looks exactly as what I need! I attached one of my sample image
Thanks a lot