I’m using fiji/ImageJ and I am trying to combine a FLIM image and a 4-channel confocal image of the same cell. First FLIM was taken while the cells was alive and after fixation I relocated the same cell with some staining. But since the images were taken with different microscopes and magnitudes, some arranging needs to be done before merging. I have done the scaling, made both the images approximately same to be fit perfectly when merged(arranged the angle, width,height etc.) but I cannot merge these images together. For instance FLIM image needs to be moved 100 micron to the left on the x axis and then added as a 5th channel to the 4-channel stack image. It would be great if you could help me on that!
One other thing is that (I can live without it but would be perfect if we can solve), when I select “merge channels” and merge 4 images into a stack and select “make a composite”, there are 4 channels in a stack but in each channel I see all those images merged together. But I would like to see each image separately and only see the merged image as another channel instead. Is that possible?
Hope I am clear with what I mean…
Thank you very much in advance!