I’m using a pipeline on my Windows 64-bit machine. The first analysis module of the pipeline is IdentifyPrimaryObjects. I’m trying to identify nuclei with this, but every time I run the module, it comes out with a streak of lines over my cells instead of circles around the nuclei.
Someone else ran the exact same pipeline on her computer (Mac 64bit) with the same images and it worked perfectly.
I’m imaging NIH 3T3 fibroblast cells. The goal of the entire pipeline is to outline cells and measure antibody fluorescence intensity. But I can’t get past the first step!
Here’s the pipeline:antibodyfluorescence.cpproj (96.2 KB)