When I open newer .dv files (from a DeltaVision Elite) the brightness and contrast is automatically set to values from -32768 to 32767, despite having no negative intensity values in my image (and a max value of at most 32767, typically less). FIJI shows it to be a 16 bit image, and nothing changes if I further try to select image-> type->16 bit.
A bigger problem is when I try to subtract background from an image (e.g. rolling ball of 50), the result is completely negative pixel values. Adding a value of 32768 to the entire image brings my image up to where I would expect intensities to be, but I am concerned that this bug may be affecting underlying data and could affect results after further processing, thus I do not want to blindly compensate for it.
This problem has persisted over 3 different window-based FIJI installations, but only appears to affect newer .dv files (I tested data from May 2018 and it opened and subtracted background without issue). It affects the raw data, as well as files that have been fused or stitched in Softworx, but not files that have been deconvolved in Softworx.
As the files are quite large, they aren’t attached directly, but I have included a screenshot showing the image and B&C when it is first opened, a single slice that was background subtracted, and measurements of the intensities before and after. Sample data (and dv log files) I have uploaded to a shareable folder on box.com. I suggest looking at 20200923_HeLa_CMG_Hoechst_60x_1520_DV_01_R3D.dv as a start.
Thanks for any help in sorting this out or working around it!