I’m looking at particulate matter deposition on various species of vegetation. In line with methodologies from various papers, I am using the auto-threshold plug-in (https://imagej.net/Auto_Threshold). The papers also state that they used the watershed feature in order to separate overlapping particles. Unfortunately, when I do this my watershed output (below) comes out with ‘catchment-style’ lines removing the particles altogether. Does anybody know why I might be having this problem?. If I use the standard threshold tool and the watershed function, everything works totally fine but this introduces a degree of human error I would rather avoid. Thanks.