Problem with 3D object counter

Hi, I’m having a problem with the 3D Object counter tool. I´m working with Z-stacks of around 0,5 Gigabyte, and I’ve noticed that some objects present in the original image, do not show up in the object map, even though their fluorescence intensity is way above the threshold. It is not a problem of size because similar object appear in the object map.

what can I do??

Thanks a lot

Hi @Maria_Victoria_Pepe,

sorry that this has been sitting unanswered for so long. Can you provide an example image that would allow to reproduce this problem?

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