thank you for your fast reply. Indeed I didn’t recognized, that during tubeness filter the slices merged into channels. I used the following jython code.
Thank you for this note, no I can go on :).
#@ Integer sigma1
#@OUTPUT Dataset output
#@ OpService ops
#@ DatasetService ds
#@ ConvertService convertService
from net.imagej import Dataset
from ij import WindowManager as wm
img = wm.getCurrentImage()
data = convertService.convert(img, Dataset)
tube = ops.filter().tubeness(data,sigma1)
# Create output Dataset
output = ds.create(tube)