hi again. I’m working with a stack of 160nm thickness, wich after some calculations amounts to 9.38pixels.
now, when I set the thickness of my z-stack in TrakEM2 and try to do a 3d viewing of the areas I marked, what I get is this:
Snapshot.tif (2.2 MB)
where either the thickness is doubled to each side or the distance between layers is halved. aniway, the problem is they overlap, and when I go to set z-layer and thickness it only allows me to set a scale, but not change them individually.
someone knows what I’m doing wrong?
Thanks for your time