I am a new user to cellprofiler and am having difficulties creat a pipeline that can measue bands in meat fibres.
I have attached an image below so that you can see what I am talking about. The consist of several meat myofibrils which are thread like structures that have two different types of band. I want to measure the width of both types of band.I have attached the pipeline which I had tried- not sure why its doesnt work.
I am not sure how to set an accurate threshold, as when I’m measuring after I cliack on the image a line doesnt appear between the two endpoints.
I have also treid to use the enhance edges module but find it turn my images totally black.
Any help you could give me would be greatly appreciated.
2010_11_22.cp (7 KB)