Problem identifying nuclei as primary objects

Hi, I am trying to quantitate a nuclear translocation assay. It looks like the dapi staining is a bit punctate and so cell profiler is having difficulty finding the nuclear edges. The result is that each nucleus is broken into a few fragments. Any suggestions on how to sort of fuzz the region so that it generates the correct nuclear region (happy to attach images but can seem to do it)
thanks for the great application !,
Bob

Hi Bob,
You can insert at “Smooth” module upstream of your IdentifyPrimaryObjects module to smooth the images.
Good luck!