First of all thanks for nice software for HCS data analysis.
I am new to forum and have started using Cell profiler recently. I have one problem. I am trying to segment mitochondria and while setting up primary automatic segmentation, if I am setting
- Try to mergee small objects with the nearby large object= Yes
Its giving some kind of error which is shown in C:\ prompt cell profiler.exe
It stops and does not segments then further? If I do not allow merging it can segment.
Secondly sometimes I have issues with memory (though my laptop has 2 GB RAM).
Can you advise me what should I do?
Thanks and regards