This is my first visit to this site, so please let me first introduce myself. My name is David Schwarz and my labs discipline is immuno-oncology. Our current interest is macrophage development and function, and we recently acquired an Etaluma LS560 to enable real-time video microscopy. The first projects have been cytokine-driven in vitro macrophage differentiation from human monocytes. We have quite decent data sets now, and we would like to evaluate those data so that we can assign measurable indices to the biologies we have observed.
Our monocytes begin life as a somewhat round cell, but over the course of several days they will change shape - sometimes quite dramatically - as they migrate around the plate. My question - is there software beyond trackmate that will allow us to isolate a subset of cells in the field (5-10), assign them each a unique color, and then track their various movements over time? I’m looking to track not only overall movement, but also changes in shape and polarity.
We are at best new to ImageJ, and at worst the annoying new user asking questions many times addressed in the forum - but not knowing the best key words to find the answers we seek. We’re not looking for someone to hold our hand through this, only a simple “yes, there is software for this”, or “you aren’t the first to want to do this, but at present there isn’t a consolidated software package - I’m afraid you’ll have to do it manually”.
Thanks for reading and any assistance you can offer.