Hey! New user here - been reading up on identifying objects but can’t seem to find what I want, which is to define an area within which I want to identify objects/cells.
Basically I’ve got the pipeline to idenfify a tertiary object (like so:https://blog.cellprofiler.org/2016/12/21/help-what-are-these-three-different-modules-to-identify-objects/) (except there’s just one object, always, not multiple) and what I want is to then identify objects again within that defined area, “cytoplasm”. Like if they were vesicles, or something.
Is there anyway to do this?
Thanks for the help!