I’m trying to detect DAB in H-DAB staining and in my case the protein is expressed and diffused in the cytoplasm. When I run the positive cell detection command, I see the software smartly segment the cells somehow which is not super accurate, sometimes it consider one big cell as some small ones and the other way round. How can I make sure what value for cell expansion I need to choose to be accurate. I excluded the cell nucleus by the way and I’m detecting Cell: DAB OD mean.
Thanks in advance