I am having a bit of trouble including metadata pertaining to the control status of images, using LoadData and for use in CalculateStatistics.
I currently have a LoadData CSV file that follows the layout below:
FileName_DAPI FileName_FITC FileName_TxRd ImageNumber Metadata_Site Metadata_Well Metadata_Control PathName_DAPI PathName_FITC PathName_TxRd
With Metadata_Control containing either -1,0 or 1.
The “Where is the information about the positive and negative control status of each image?” section of the CalculateStatistics module is highlighted in red. Hovering over this returns the error, “Metadata has an unmeasured feature name”.
From reading through the manuals I can’t see where I am going wrong. From what I can garner, the layout of the LoadData file in order to include control information is correct. Am I mistaken? If so can anyone offer any advice.
I have attached the LoadData.CSV file and the pipeline.