I am attempting to use the polygon tool to select a region in an image that was taken on an Aperio slide scanner. The images are saved as SVS files. The Bio-Formats Importer plugin has been used to read these files into Fiji. Application of the polygon tool to the resulting image series causes distortion of the images, as depicted in the attached file.
I have tried restarting Fiji; restarting my computer; and re-installing the Bio-Formats plugin. These steps have failed to correct image distortion.
Has anyone encountered this problem before? What might be a viable solution?
I would be grateful for any insights that you are able to lend!